The has been designed with an open architecture and chemistry format that allows user flexibility in qPCR methods and research pursuits. The supporting Quansoft® software provides an easy to use comprehensive data analysis package with the flexibility to edit experiment setup even after the run is complete.
PrimeQ Instrument
Key Features
SINGLE WHITE LED LIGHT SOURCE
- Ensures consistent power output to each well compared to tungsten halogen lamps or other multiple LED systems.
- Provides a long term life span unlike tungsten halogen lamps that require frequent replacement.
PMT (PHOTOMULTIPLIER TUBE) DETECTOR
- PMT is designed for accurate detection of photons compared to CCD cameras which are just an imaging system.
- PMT provides raw data for analysis that can be manipulated directly whereas CCD uses image comparisons interpreted by internal algorithms.
FLEXIBLE SCANNING MECHANISM
- Allows both partial and full plate reads.
- High accuracy ensures no cross talk between well reads.
- Full plate read in 20 seconds per filter.
MULTIPLEX CAPABILITIES
- 4 paired excitation and emission filters housed in an individual cartridge system.
- Open system to preferred chemistry and not locked in to factory calibrated dyes.
HEATED LID
- The flexible optical heated lid system is designed to minimize loss of sample and prevent sample condensation.
- Temperature controlled, it allows further method optimization where pre-incubation stages may be required (e.g. in reverse transcription techniques) or where changes in the heated lid characteristics are needed during the different stages of a program run.
Quansoft® software and reporting
Accompanying PrimeQ is our easy-to-use, flexible software Quansoft®, which gives you the freedom to manipulate your raw data, choose your own chemistries and consumables.
Key Features
- Ability to create, modify and save files using Quansoft® while a program is running on the unit
- Ability to run without initially defining your plate layout and analysis criteria - just pop a prepared plate in & run a program
- Ability to export unprocessed, raw data
- Install Quansoft® on multiple PCs without paying a license fee (only a simple registration is required)
- Ability to change from first order derivative to fit points in results analysis
Technical Specifications
| Parameter | PrimeQ |
|---|---|
| Block format | 96 x 0.2ml |
| Block specification | 8 x peltier elements per block. Quad-circuit technology to enhance performance |
| Block uniformity at 50°C | ± 0.25°C |
| Maximum ramp rate | Up to 2.2°C |
| Temperature range | 4 to 98°C |
| Sample volume | 15 to 50ul |
| Heated lid | Adjustable between 100 and 115°C in 1°C increments, or off |
| Maximum sample evaporation | Less than 15 volume loss from any well using the plate sealing recommended by Techne |
| PC connectivity | USB, OS Win XP SP3 or higher and Win 7 (one unit per PC) |
| Plate format | Low profile 96-well plate (skirted or non-skirted) |
| Excitation source | Solid state white light source |
| Detector | Photon counting photomultiplier tube |
| Multiplex dye detection | Up to 4 dyes per reaction tube |
| User chosen filters | Maximum of 4 paired excitation emission filter cartridge systems suitable wth currently used dyes* |
| Fluorescence excitation range | 470-650nm (standard filters are centered at 485, 530, 580 and 640nm) |
| Fluorescence detection range | 500-710nm (standard filters are centered at 520, 560, 615 and 685nm) |
| Dynamic range | At least 9 orders of magnitude of target DNA concentration |
| Sensitivity (detection threshold) | 1nM fluorescein in a 20ul sample |
| Resolution | 1.5 fold difference in target DNA concentration |
| Weight | 25kg |
| Dimensions (WxHxD) | 407 x 508 x 534mm |
| Input power | 715W |
| Power supply | 100-230V, 50-60Hz |
*custom filter cartridge systems available upon request
Ordering Information
| Product Code | Description |
| PrimeQ | |
| FC01* | FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength |
| FC02 | Green, FAM|SYBR®, 485nm excitation wavelength, 520nm emission wavelength |
| FC03 | Yellow, HEX, 530nm excitation wavelength, 560nm emission wavelength |
| FC04 | Red, ROX, 580nm excitation wavelength, 615nm emission wavelength |
| FC05 | Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength |
*Please note FC01 is not included with the unit. FC02-FC05 are included with the unit
Bibby reserve the right to make specification changes at any time.
However, the information on this website is correct at time of reading.